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M9480865.TXT
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1994-09-05
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Document 0865
DOCN M9480865
TI Synergistic antiretroviral activities of the herb, Prunella vulgaris,
with AZT, ddI, and ddC.
DT 9410
AU John JF; Kuk R; Rosenthal A; Robert Wood Johnson Medical School, New
Brunswick, N.J.
SO Abstr Gen Meet Am Soc Microbiol. 1994;94:481 (abstract no. S-27). Unique
Identifier : AIDSLINE ASM94/94313080
AB There is a urgent need for new, safe, and inexpensive therapy for HIV
infection. We tested a group of commonly available herbs for their
activity in aqueous solution against HIV. In screening tests of four
commonly used herbs, Prunella vulgaris (PV) was found to exert the best
anti-HIV activity. The antiretroviral activity of PV was further
analyzed using an aqueous extract prepared by boiling 3 gm in 100 ml.
water for 45 min. followed by filter sterilization. The ability of
different dilutions of PV to inhibit replication of HIV strain H9/3B was
monitored by inhibition of HIV-induced cytotoxicity in MT2 cells,
measured by the MTT uptake assay. HIV inhibition of PV alone was tested
in four dilutions (undiluted, 1:10, 1:100, and 1:1000). MT2 cell
cytotoxicity was present at undiluted and 1:10 dilutions. The 1:100 and
1:1000 dilutions gave 80-90% and 0-25% protection respectively. PV was
next tested in combination with AZT, ddI, or ddC. The combinations of PV
(1:1000) plus AZT (0.003-0.01 microM) and PV plus ddI (0.1-0.3 microM)
gave protection of 69-74% compared to a much lower protection range
(10%) by AZT or ddI alone at the same concentrations. Only a slight
additive effect was observed for PV plus ddC. We conclude that certain
herbs like PV may display synergistic anti-HIV activity when used in
combination with antiretroviral nucleosides.
DE Cell Line Comparative Study Didanosine/*TOXICITY Dose-Response
Relationship, Drug Drug Synergism *Herbs Human HIV/*DRUG
EFFECTS/PHYSIOLOGY Plant Extracts/*TOXICITY Virus Replication/*DRUG
EFFECTS Zalcitabine/*TOXICITY Zidovudine/*TOXICITY MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).